polymerase chain reaction
- polymerase chain reaction
-
The first practical system for
in vitro amplification of DNA, and as such one of the most important recent developments in molecular biology. Two synthetic
oligonucleotide primers, which are complementary to two regions of the target
DNA (one for each strand) to be amplified, are added to the target
DNA (that need not be pure), in the presence of excess deoxynucleotides and
Taq polymerase,
a heat-stable
DNA polymerase. In
a series (typically 30) of temperature cycles, the target
DNA is repeatedly denatured (around 90°C), annealed to the primers (typically at 50-60°C) and
a daughter strand extended from the primers (72°C). As the daughter strands themselves act as templates for subsequent cycles,
DNA fragments matching both primers are amplified exponentially, rather than linearly. The original
DNA need thus be neither pure nor abundant, and the
PCR reaction has accordingly become widely used not only in research, but in clinical diagnostics and forensic science.
Dictionary of molecular biology.
2004.
Look at other dictionaries:
Polymerase Chain Reaction — Réaction en chaîne par polymérase Pour les articles homonymes, voir PCR. Diagramme des 4 premiers cycles de la PCR La réaction en chaîne par polymérase (PCR est l abréviation an … Wikipédia en Français
polymerase chain reaction — n. a technique in which a known DNA sequence is synthesized at high temperatures by means of a polymerase, producing millions of copies for statistical analysis: used in DNA fingerprinting, in detecting minute quantities of cancer cells, etc. * * … Universalium
polymerase chain reaction — n an in vitro technique for rapidly synthesizing large quantities of a given DNA segment that involves separating the DNA into its two complementary strands, binding a primer to each single strand at the end of the given DNA segment where… … Medical dictionary
polymerase chain reaction — n. a technique in which a known DNA sequence is synthesized at high temperatures by means of a polymerase, producing millions of copies for statistical analysis: used in DNA fingerprinting, in detecting minute quantities of cancer cells, etc … English World dictionary
polymerase chain reaction — polymerase chain reaction. См. полимеразная цепная реакция. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
Polymerase chain reaction — PCR redirects here. For other uses, see PCR (disambiguation). A strip of eight PCR tubes, each containing a 100 μl reaction mixture The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a… … Wikipedia
Polymerase Chain Reaction — Die Polymerase Kettenreaktion (englisch Polymerase Chain Reaction, PCR) ist eine Methode, um die Erbsubstanz DNA in vitro zu vervielfältigen. Dazu wird ein Enzym verwendet, die DNA Polymerase. Der Begriff Kettenreaktion beschreibt in diesem… … Deutsch Wikipedia
polymerase chain reaction — polimerazinė grandininė reakcija statusas T sritis augalininkystė apibrėžtis DNR fragmentų dauginimas in vitro. atitikmenys: angl. PCR; polymerase chain reaction rus. полимеразная цепная реакция; ПЦР ryšiai: sinonimas – PGR … Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas
Polymerase chain reaction optimization — The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization have been developed by molecular biologists to improve PCR performance and minimize failure. Contents 1… … Wikipedia
Polymerase Chain Reaction — Po|ly|me|ra|se Chain Re|ac|ti|on [pɔ lɪməreɪz tʃeɪn rɪ ækʃn; engl. = Polymerase Kettenreaktion]: svw. ↑ PCR … Universal-Lexikon
